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Dhanjal, Daljeet Singh
- Toxicological Effects of Lambda-Cyhalothrin on Liver, Kidney and Testis of Indian Catfish Clarias batrachus
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Authors
Jasdeep Singh
1,
Simranjeet Singh
2,
Shivika Datta
3,
Joydeep Dutta
3,
Daljeet Singh Dhanjal
2,
Anchal Saini
2,
Joginder Singh
2
Affiliations
1 Department of Bioscience, Barkatullah University, Bhopal, Madhya Pradesh, IN
2 Department of Biotechnology, Lovely Professional University, Phagwara, Punjab, IN
3 Department of Zoology, Lovely Professional University, Phagwara, Punjab, IN
1 Department of Bioscience, Barkatullah University, Bhopal, Madhya Pradesh, IN
2 Department of Biotechnology, Lovely Professional University, Phagwara, Punjab, IN
3 Department of Zoology, Lovely Professional University, Phagwara, Punjab, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 3 (2015), Pagination: 128-136Abstract
Aquatic organisms come in direct contact with the pesticides that sprayed onto the agricultural lands are washed out by the rain water. Fishes are affected by exposure to these pesticides. The present study was conducted to determine the toxicity of lambda cyhalothrin on the kidney, liver & testis and biochemical, hematological, behavioural parameters of Clarias batrachus. Control group fed with normal diet and kept for 15 and 30 days. The treated groups included normal diet along with 1.33 μg L-1 of Lambda-cyhalothrin for 15 days and 30 days. The treatment group exhibited disrupted shoaling behaviour characterized by irregular, erratic, and darting swimming movements and loss of equilibrium. Administration of lambda cyhalothrin also causes damage to the tissues of the testes, kidney and liver. Marked changes in the histo-architecture of these organs were observed. All these results suggest that the lambda cyhalothrin induced changes in C. batrachus directly or indirectly by modulating the physiological functions in the fishes.Keywords
Insecticide, Behavioural Studies, Histopathology, Haematology, Enzymology.References
- Stoytcheva M. Pesticides in the modern world-risks and benefits. Role of Pesticides in Human Life in the Modern Age. Intech open access publisher; 2011.
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- Singh S, Kashyap N, Singla S, et al. Bioremediation of heavy metals by employing resistant microbial isolates from agricultural soil irrigated with Industrial Waste water. Oriental J Chem 2015;31(1):357-361.
- Kumar V, Singh S, Manhas A, et al. Bioremediation of petroleum hydrocarbon by using Pseudomonas species isolated from petroleum contaminated soil. Oriental J Chem 2014;30(4):1771-1776.
- Kumar V, Upadhyay N, Kumar V, et al. A review on sample preparation and chromatographic determination of acephate and methamidophos in different samples. Arab J Chem 2015a doi:10.1016/j.arabjc.2014.12.007.
- Kumar V, Upadhyay N, Kumar V, et al. Environmental exposure and health risks of the insecticide monocrotophos: a review. J Biodivers Environ Sci 2014;5:111–120.
- Kumar V, Upadhyay N, Singh S, Singh J, Kaur P Thin-layer chromatography: comparative estimation of soil’s atrazine. Curr World Environ 2013b;8:469–473.
- Kumar V, Upadhyay N, Wasit AB, et al. Spectroscopic methods for the detection of organophosphate pesticides: a preview. Curr World Environ 2013a;8(2):313–318.
- Saha S., Kaviraj A. Acute Toxicity of Synthetic Pyrethroid Cypermethrin to Some Freshwater Organisms. Bull Environ Contam Toxicol 2008;80:49-52.
- Yousef MI. Vitamin E modulates reproductive toxicity of pyrethroid lambda-cyhalothrin in male rabbits. Food Chem Toxicol 2010;48:1152-1159.
- Chowdhury A, Pradhan S, Saha M, et al. Impact of pesticides on soil microbiological parameters and possible bioremediation strategies. Ind J Microbiol 2008;48:114-127.
- Piner P, Üner N. Neurotoxic effects of lambda‐cyhalothrin modulated by piperonyl butoxide in the brain of Oreochromis niloticus. Environ Toxicol 2014;29:1275-1282.
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- Gunasekaran, K., Sahu, S. S., Vijayakumar, T., Subramanian, S., & Jambulingam, P. Effect of house spraying with lambdacyhalothrin 10 per cent capsule suspension (CS) formulation in comparison with 10 per cent wettable powder (WP) against malaria vector in Malkangiri district, Odisha, India. The Ind J Med Res 2014;140(4):538.
- Velmurugan, B., Selvanayagam, M., Cengiz, E. I., & Unlu, E. Histopathology of lambda-cyhalothrin on tissues (gill, kidney, liver and intestine) of Cirrhinus mrigala. Environ Toxicol Pharma 2007;24(3):286-291.
- Kumar A, Sharma B, Pandey RS. Assessment of acute toxicity of λ-cyhalothrin to a freshwater catfish, Clarias batrachus. Environ Chem Lett 2011;9(1):43-46.
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- Yadav RP, Singh D, Singh SK, Singh A. Metabolic changes in Freshwater Fish Channa punctatus due to stem-bark extract of Croton tiglium. Pak J Biol Sci 2003;6:1223-1228.
- Kumar, A., Rai DK, Sharma B, et al. λ-cyhalothrin and cypermethrin induced in-vivoalterations in the activity of acetylcholinesterase in a freshwater fish, Channa punctatus ( Bloch). Pest Biochem Physiol 2009;93:96–99.
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- Isolation and Characterization of Microbial Diversity of Soil of Dhanbad Coal Mines using Molecular Approach
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Authors
Affiliations
1 School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, IN
1 School of Bioengineering and Biosciences, Lovely Professional University, Punjab-144411, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1137-1140Abstract
Soil is the richest ecosystem there is, as far as the diversity and populations of microorganisms is concerned. It is known that there could be over ten billion microbes per gram of soil, making soil the ideal ecosystem for mining different genes, ORFs etc. Microbial Diversity forms the lifeline of an ecosystem, interfering, regulating and participating in the biogeochemical activities therein. The present study was conducted to identify key extremophilic species residing in the coal mines of Dhanbad plateau in Bihar, India. Dhanbad plateau soil presents a unique niche, rich in coal and minerals. Soil from the coal mines was analysed through metagenomic as well as culture-dependent approach to enlist the micro-organisms native to the place. The culturable fraction lead to the identification of Klebsiella spp, Enterobacteriaceae, Serratia marcescens, and Providencia spp.Keywords
Microbial Diversity, Molecular techniques, 16S rRNA genes, Dhanbad Plateau, Metagenomics.References
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- Accessing Genetic Diversity and Phylogenetic Analysis of Microbial Population of Soil from Hygam Wetland of Kashmir Valley
Abstract Views :171 |
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Authors
Mousumi Sarangi
1,
Chirag Chopra
1,
Yusuf Ado Usman
2,
Daljeet Singh Dhanjal
1,
Reena Singh Chopra
1
Affiliations
1 School of Bioengineering and Biosciences, Lovely Professional University, Phagwara -144411, IN
2 Department of Biochemistry, Faculty of Basic Medical Science, Bayero University Kano, NG
1 School of Bioengineering and Biosciences, Lovely Professional University, Phagwara -144411, IN
2 Department of Biochemistry, Faculty of Basic Medical Science, Bayero University Kano, NG
Source
Research Journal of Pharmacy and Technology, Vol 12, No 5 (2019), Pagination: 2323-2326Abstract
Assessing microbial diversity of a region is important for understanding ecosystem functions and processes. The cold wetlands of the Himalayas are relatively unexplored for the microbial diversity. In the present study, one such wetland of the Kashmir region, Hygam Wetland (34°13′30′′- 34°16′4′′N and 74°30′27′′- 74°32′33′′E), was chosen for determination of the microbial diversity. Metagenomic DNA was extracted and 16S r-RNA gene was amplified followed by sequencing and BLASTn analysis of the sequences. Phylogenetic tree of identified sequences was made by pairwise alignment followed by tree generation using Clustal omega. In the BLASTn analysis, similarity with Bacillus safensis, uncultured Lysinibacillus C1A14-2 and uncultured clone 4015B10 was observed. Further, the microbes cultured from Hygam soil were identified using biochemical tests. These included Shigella species, Salmonella choleraesuis, Citrobacter species and Escherichia coli.Keywords
Hygam, Kashmir Wetland, Metagenomics, Microbial Diversity, Phylogenetic Analysis.References
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